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Detection and Quantification of Calcitonin Gene-Related Peptide (CGRP) in Human Plasma Using a Modified Enzyme-Linked Immunosorbent Assay
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Biochimie
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Journal JoVE Biochimie
Detection and Quantification of Calcitonin Gene-Related Peptide (CGRP) in Human Plasma Using a Modified Enzyme-Linked Immunosorbent Assay

Detection and Quantification of Calcitonin Gene-Related Peptide (CGRP) in Human Plasma Using a Modified Enzyme-Linked Immunosorbent Assay

DOI:
10.3791/64775-v

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07:14 min

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June 16, 2023

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, , , , , , , ,
1Department of Otolaryngology-Head & Neck Surgery,Johns Hopkins University School of Medicine, 2Virginia Commonwealth University School of Medicine, 3Department of Oncology, The Sidney Kimmel Comprehensive Cancer Center,Johns Hopkins University School of Medicine, 4Department of Otolaryngology-Head and Neck Surgery,University of Michigan Medical School, 5Department of Otolaryngology-Head and Neck Surgery,The Ohio State University College of Medicine, 6ICTR Clinical Research Core Laboratory,Johns Hopkins University School of Medicine

Chapitres

  • 00:04Introduction
  • 01:03Collection, Preparation, and Extraction of Plasma Sample
  • 03:28ELISA Plate Preparation (Blocking)
  • 04:16Enzyme-Linked Immunosorbent Assay (ELISA)
  • 06:06Results: Effect of Blocking on CGRP Quantification
  • 06:29Conclusion

Summary

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Traduction automatique

Published data pertaining to calcitonin gene-related peptide (CGRP) concentrations in human plasma are inconsistent. These inconsistencies may be due to the lack of a standardized, validated methodology to quantify this neuropeptide. Here, we describe a validated enzyme-linked immunosorbent assay (ELISA) protocol to purify and quantify CGRP in human plasma.

Tags

CGRPEnzyme-linked Immunosorbent AssayELISAPlasmaPurificationExtractionQuantificationMigraineBiomarker

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