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1Department of Otolaryngology-Head & Neck Surgery,Johns Hopkins University School of Medicine, 2Virginia Commonwealth University School of Medicine, 3Department of Oncology, The Sidney Kimmel Comprehensive Cancer Center,Johns Hopkins University School of Medicine, 4Department of Otolaryngology-Head and Neck Surgery,University of Michigan Medical School, 5Department of Otolaryngology-Head and Neck Surgery,The Ohio State University College of Medicine, 6ICTR Clinical Research Core Laboratory,Johns Hopkins University School of Medicine
Chapitres
- 00:04Introduction
- 01:03Collection, Preparation, and Extraction of Plasma Sample
- 03:28ELISA Plate Preparation (Blocking)
- 04:16Enzyme-Linked Immunosorbent Assay (ELISA)
- 06:06Results: Effect of Blocking on CGRP Quantification
- 06:29Conclusion
Offentliggjorte data vedrørende calcitonin gen-relaterede peptid (CGRP) koncentrationer i humant plasma er inkonsekvente. Disse uoverensstemmelser kan skyldes manglen på en standardiseret, valideret metode til kvantificering af dette neuropeptid. Her beskriver vi en valideret enzymbundet immunosorbentassay (ELISA) protokol til oprensning og kvantificering af CGRP i humant plasma.
