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Real-Time Quantification of the Effects of IS200/IS605 Family-Associated TnpB on Transposon Activity
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Biologie
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Journal JoVE Biologie
Real-Time Quantification of the Effects of IS200/IS605 Family-Associated TnpB on Transposon Activity

Real-Time Quantification of the Effects of IS200/IS605 Family-Associated TnpB on Transposon Activity

DOI:
10.3791/64825-v

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04:04 min

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January 20, 2023

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, ,
1Department of Physics and Astronomy,University of California, 2Microbiology Program,University of California, 3Biophysics Program,University of California

Chapitres

  • 00:05Introduction
  • 00:44Slide Preparation
  • 01:59Timelapse Fluorescence Microscopy
  • 02:25Results: Visualizing Transposon Activity in Live E. coli Cells
  • 03:30Conclusion

Summary

Traduction automatique

Traduction automatique

A protocol is outlined to perform live real-time imaging to quantify how the accessory protein TnpB affects the dynamics of transposition in individual live Escherichia coli cells.

Tags

Real-time QuantificationIS200/IS605 FamilyTnpBTransposon ActivitySingle-cell AnalysisLive Cell ImagingFluorescence MicroscopyExcision DynamicsTransposaseM63 Agarose PadBacterial CultureEpoxy SealTime-lapse Imaging

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