Journal
/
Immunologie et infection
/
Gene Editing of Primary Rhesus Macaque B Cells
Journal JoVE
Immunologie et infection
This content is Free Access.
Journal JoVE Immunologie et infection
Gene Editing of Primary Rhesus Macaque B Cells

Gene Editing of Primary Rhesus Macaque B Cells

DOI:
10.3791/64858-v

•

09:53 min

•

February 10, 2023

•

, , , , , , , ,
1Laboratory of Molecular Immunology,The Rockefeller University, 2Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases,National Institutes of Health, 3Laboratory of Retrovirology,The Rockefeller University, 4Howard Hughes Medical Institute,The Rockefeller University, 5Laboratory of Applied Virology and Precision Medicine,King Abdullah University of Science and Technology (KAUST), 6Vaccine and Immunotherapy Center,Wistar Institute

Chapitres

  • 00:04Introduction
  • 00:48Preparation and Culture of Rhesus Macaque B Cells
  • 02:39Primary Rhesus Macaque B Cell Gene Editing
  • 07:36Results: Genome Editing of Primary Rhesus Macaque B Cells
  • 09:22Conclusion

Summary

Traduction automatique

Traduction automatique

We present a method for culturing and gene editing primary rhesus macaque B cells using CRISPR/Cas9 and recombinant adeno-associated virus serotype 6 for the study of B cell therapies.

Tags

Gene EditingPrimary Rhesus Macaque B CellsCell TherapyB Cell CultureCRISPR-Cas9RAAV6Homology-directed RepairCell ActivationCell PreparationElectroporationTransductionPreclinical Research

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

Murine Model of CD40-activation of B cells

Multicolor Flow Cytometry Analyses of Cellular Immune Response in Rhesus Macaques

Induction and Assessment of Class Switch Recombination in Purified Murine B Cells

Recombinant Retroviral Production and Infection of B Cells

Preparation and Use of HIV-1 Infected Primary CD4+ T-Cells as Target Cells in Natural Killer Cell Cytotoxic Assays

Generation of Multivirus-specific T Cells to Prevent/treat Viral Infections after Allogeneic Hematopoietic Stem Cell Transplant

piggyBac Transposon System Modification of Primary Human T Cells

Detection of True IgE-expressing Mouse B Lineage Cells

Generation of Recombinant Human IgG Monoclonal Antibodies from Immortalized Sorted B Cells

Generating Recombinant Avian Herpesvirus Vectors with CRISPR/Cas9 Gene Editing

Read Article