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Microvascular and Macrovascular Endothelial Cell Isolation and Purification from Lung-Derived Samples
Journal JoVE
Biologie
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Journal JoVE Biologie
Microvascular and Macrovascular Endothelial Cell Isolation and Purification from Lung-Derived Samples
DOI:

05:26 min

February 03, 2023

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Chapitres

  • 00:04Introduction
  • 00:45Sample Preparation and Enzymatic Digestion
  • 01:47Recovery of the Digested Cells and Removal of Non-Endothelial Cells (EC) by Filtration
  • 02:32Sedimentation of Cell Clusters and Cell Seeding
  • 03:16Results: Purification and Characterization of Freshly Isolated HLMVECs
  • 04:30Conclusion

Summary

Traduction automatique

Although challenging, the isolation of pulmonary endothelial cells is essential for studies on lung inflammation. The present protocol describes a procedure for the high-yield, high-purity isolation of macrovascular and microvascular endothelial cells.

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