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Chapitres
- 00:04Introduction
- 01:12Establishing Breast Tumor/Normal Organoids from Resected Tissue
- 03:59Passaging and Expanding Patient-Derived Breast Organoids in Culture
- 06:39Results: Morphology and Growth Rate of Patient-Derived Breast Tumor Organoid Lines
- 07:39Conclusion
A detailed protocol is provided here for establishing human breast organoids from patient-derived breast tumor resections or normal breast tissue. The protocol provides comprehensive step-by-step instructions for culturing, freezing, and thawing human patient-derived breast organoids.
