Journal
/
/
光控生物活性化合物的体外体内评价 - 癌症光药理学的潜在候选药物
Journal JoVE
Chimie
Author Produced
This content is Free Access.
Journal JoVE Chimie
In Vitro and In Vivo Evaluation of Photocontrolled Biologically Active Compounds – Potential Drug Candidates for Cancer Photopharmacology
DOI:

13:17 min

September 29, 2023

, , , , , , , , , , , ,

Chapitres

  • 00:00Introduction
  • 01:22IC50 Evaluation for LMB002 (“ring-closed” and “ring-open” forms) Using 2D and 3D LLC Cell Cultures
  • 03:062D Cell Culture Experiment—Adding the Compounds (Day 2)
  • 04:142D Cell Culture Experiment—Staining and Imaging (Days 2–5)
  • 04:453D Cell Culture Experiment
  • 05:46Determination of Photoswitching Efficiency in a Tissue Surrogate
  • 07:46In Vivo Anticancer Efficacy Determination
  • 09:39Representative Results
  • 12:40Conclusion

Summary

Traduction automatique

该协议提供了一组用于评估可光开关抗癌肽的实验,可用于此类化合物的临床前筛选。这包括 2D 和 3D 细胞培养物中的细胞毒性评估、 离体 (模型组织)光异构化效率和 体内 功效的评估。

Vidéos Connexes

Read Article