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Demonstration of the DNA Fiber Assay for Investigating DNA Damage and Repair Dynamics Induced by Nanoparticles
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Biologie
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Journal JoVE Biologie
Demonstration of the DNA Fiber Assay for Investigating DNA Damage and Repair Dynamics Induced by Nanoparticles

Demonstration of the DNA Fiber Assay for Investigating DNA Damage and Repair Dynamics Induced by Nanoparticles

DOI:
10.3791/64903-v

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13:09 min

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March 03, 2023

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, ,
1Department of Biomedical Sciences,University of Illinois College of Medicine Rockford, 2Department of Health Sciences Education,University of Illinois College of Medicine Rockford

Chapitres

  • 00:04Introduction
  • 02:05Preparation for the Fiber Assay
  • 06:42Performing the DNA Fiber Assay and Image Acquisition
  • 10:33Results: Analysis of Variation in DNA Replication Dynamics in the Presence of Nanoparticles
  • 12:04Conclusion

Summary

Traduction automatique

Traduction automatique

The methodology assists in the analysis of variation in DNA replication dynamics in the presence of nanoparticles. Different methodologies can be adopted based on the cytotoxicity level of the material of interest. In addition, a description of the image analysis is provided to help in DNA fiber analysis.

Tags

DNA Fiber AssayDNA DamageDNA RepairNanoparticlesDNA Replication DynamicsChromatin DynamicsTherapeutic AgentsNanomedicineRAW 264.5 Macrophage CellsIododeoxyuridineChloro-iododeoxyuridine

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