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Isolating and Culturing Vestibular and Spiral Ganglion Somata from Neonatal Rodents for Patch-Clamp Recordings
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Neurosciences
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Journal JoVE Neurosciences
Isolating and Culturing Vestibular and Spiral Ganglion Somata from Neonatal Rodents for Patch-Clamp Recordings

Isolating and Culturing Vestibular and Spiral Ganglion Somata from Neonatal Rodents for Patch-Clamp Recordings

DOI:
10.3791/64908-v

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11:05 min

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April 21, 2023

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, , , , ,
1Caruso Department of Otolaryngology Head & Neck Surgery, Zilkha Neurogenetics Institute, Hearing and Communications Neuroscience Training Program,University of Southern California

Chapitres

  • 00:00Introduction
  • 01:09Fabricating Trituration Pipettes
  • 01:52Extracting the Superior Vestibular Ganglion
  • 04:01Tissue Dissociation and Plating
  • 05:24Extracting the Spiral Ganglion
  • 06:58Patch-Clamp Recordings
  • 08:31Results: Voltage-Clamp Protocols to Measure Diverse Groups of Ionic Currents and Diverse Firing Patterns
  • 10:25Conclusion

Summary

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Presented here are methods providing detailed instructions for dissecting, dissociating, culturing, and patch-clamp recording from vestibular ganglion and spiral ganglion neurons of the inner ear.

Tags

Keywords: VestibularSpiral GanglionPatch-clampNeonatal RodentsAuditory Afferent NeuronsCell BodiesIon ChannelsNeurotransmitter ReceptorsIn VitroVoltage-dependent PropertiesCell DiversityDissociationOtic CapsuleAuditory NerveSuperior GanglionInferior GanglionPeripheral Nerve BranchUtricle

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