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Lysinduceret dielektroforese til karakterisering af den elektriske opførsel af humane mesenkymale stamceller
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1Department of Chemical and Biomolecular Engineering, Samueli School of Engineering,University of California, Irvine, 2Department of Biomedical Engineering, Samueli School of Engineering,University of California, Irvine, 3Department of Chemistry, School of Physical Sciences,University of California, Irvine, 4Sue & Bill Gross Stem Cell Research Center,University of California, Irvine
Chapitres
- 00:05Introduction
- 00:50Light-Induced Dielectrophoresis (LiDEP) Chip Device Fabrication
- 03:05Dielectrophoresis (DEP) Buffer Preparation
- 03:55Cell Preparation
- 04:40Light-Induced Dielectrophoresis (LiDEP) Characterization
- 07:41Results: Dielectrophoresis Responses in hMSCs
- 09:28Conclusion
Her præsenterer vi lysinduceret dielektroforese som en etiketfri tilgang til karakterisering af heterogene cellelinjer, specifikt humane mesenkymale stamceller (hMSC'er). Dette papir beskriver en protokol til brug og optimering af en mikrofluidisk enhed med et fotoledende lag til at karakterisere hMSC'ers elektriske opførsel uden at ændre deres oprindelige tilstand.
