/

Immunologie et infection

/

通过流式细胞术同时评估造血干细胞和祖细胞的亲缘关系、分裂数和表型

Journal JoVE
Immunologie et infection

This content is Free Access.

Journal JoVE Immunologie et infection

Simultaneous Assessment of Kinship, Division Number, and Phenotype via Flow Cytometry for Hematopoietic Stem and Progenitor Cells

Please note that all translations are automatically generated. Click here for the English version.

通过流式细胞术同时评估造血干细胞和祖细胞的亲缘关系、分裂数和表型

DOI:

10.3791/64918-v

•

10:20 min

•

March 24, 2023

•

Alessandro Donada, Tamar Tak, Giulio Prevedello^2,3,4, Idan Milo, Ken R. Duffy, Leïla Perié

¹Quantitative Immuno-hematology, CNRS UMR168,Institut Curie, ²Hamilton Institute,Maynooth University, ³Institut Curie, PSL Research University, CNRS UMR 3348, Orsay, ⁴Université Paris-Sud, Université Paris-Saclay, CNRS UMR 3348, Orsay

Chapitres

00:04Introduction
00:51Cell Dye Staining
02:33Antibody Staining and Cell Sorting
05:41After Culture Antibody Staining
06:47Post‐Culture Flow Cytometry Data Analysis
08:01Results: Simultaneous Assessment of Cell Division Number, Surface Cell Phenotype, and Cellular Kinship
09:26Conclusion

Summary

Traduction automatique

这里介绍的是一种基于流式细胞术的技术，可以同时测量细胞分裂次数、表面细胞表型和细胞亲缘关系。可以使用基于排列的框架对这些属性进行统计测试。

Tags

Hematopoietic Stem Cells Hematopoietic Progenitor Cells Flow Cytometry Cell Division Cell Differentiation CFSE Labeling Cell Fate High-throughput Analysis Cell Culture Single-cell Analysis

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

免疫细胞在受伤的脊髓组织流式细胞仪定量评估：一个细胞的分离纯化方法的新颖使用

流式细胞仪分析小鼠主动脉内流的免疫细胞

人类<em在体外承认免疫失衡的早期国家禁止作为筛检工具

在纯化造血干/祖细胞识别DNA突变

神经干/祖细胞在小鼠EAE的慢性全身性注射

流式细胞仪为基础的检测方法的NK细胞功能的监控

荧光激活细胞分选从小鼠骨髓浆树突状细胞的分离纯化

A Flow Cytometry-Based Cytotoxicity Assay for the Assessment of Human NK Cell Activity

小鼠与人肺泡巨噬细胞的分离和体外培养

全骨髓的制备,用于中性粒系细胞的大规模细胞测定分析

Read Article