Journal
/
Immunologie et infection
/
实时测量中性粒细胞的线粒体生物能量特征
Journal JoVE
Immunologie et infection
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Immunologie et infection
Real-Time Measurement of the Mitochondrial Bioenergetic Profile of Neutrophils
Please note that all translations are automatically generated. Click here for the English version.

实时测量中性粒细胞的线粒体生物能量特征

DOI:
10.3791/64971-v

•

09:39 min

•

June 02, 2023

•

, ,
1Department of Immunology, School of Medicine,UConn Health, 2Arthritis and Clinical Immunology Program,Oklahoma Medical Research Foundation, 3Department of Microbiology and Immunology,University of Oklahoma Health Science Center

Chapitres

  • 00:05Introduction
  • 01:05Preparation of the 96-Well Plate for the Metabolic Extracellular Flux Assay
  • 01:52Preparation and Seeding of Cells
  • 03:52Preparing Compounds in the Mitochondrial Stress Test Kit
  • 04:25Running the Mitochondrial Stress Assay
  • 07:29Results: Metabolic Flux Assay to Analyze Oxygen Consumption Rates (OCR) in Neutrophils
  • 09:02Conclusion

Summary

Traduction automatique

Traduction automatique

我们描述了使用代谢细胞外通量分析仪测量小鼠和人中性粒细胞以及HL60细胞线粒体呼吸的逐步方案。

Tags

Mitochondrial RespirationNeutrophilsHL60 CellsMetabolic Extracellular Flux AnalyzerMitochondrial FunctionCell Seeding DensityAssay MediumMouse NeutrophilsHuman NeutrophilsDifferentiated HL60 CellsUndifferentiated HL60 CellsBackground Correction

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

一个实时，同步成像的TCR和其相关的信号转导蛋白的T​​IRF显微镜技术

T细胞实时成像实时信号复合物的形成

生物能量和氧化突发：协议用于人类白细胞和血小板的隔离与评价

胞外DNA释放的高通量测量和人中性粒定量NET形成<em>体外</em

在实时麻醉小鼠成像中性粒细胞和单核细胞在肠系膜静脉用活体显微镜

实时定量聚合酶链反应检测感染细胞培养中的登革热病毒 rna

用于口腔中性粒细胞病评价的鼠期牙周炎的强结合诱导模型

用于狂犬病诊断的泛源病毒实时RT-PCR

使用实时细胞分析监测宿主外的流感病毒存活率

中性粒细胞中活性氧物种的实时定量 感染脑膜性 大肠杆菌

Read Article