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Analysis of Extracellular Vesicle-Mediated Vascular Calcification Using In Vitro and In Vivo Models
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Biologie
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Journal JoVE Biologie
Analysis of Extracellular Vesicle-Mediated Vascular Calcification Using In Vitro and In Vivo Models

Analysis of Extracellular Vesicle-Mediated Vascular Calcification Using In Vitro and In Vivo Models

DOI:
10.3791/65013-v

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09:01 min

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January 27, 2023

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, , ,
1Department of Biomedical Engineering,Florida International University

Chapitres

  • 00:05Introduction
  • 00:54Organ Removal
  • 01:50Bone Removal
  • 02:50Microdissection
  • 04:48Isolating and Extracting Extracellular Vesicles from Aortas
  • 05:26Assessing the Calcification Potential of Extracellular Vesicles with Light Scattering Absorbance
  • 06:11Assessing the Calcification Mineral Formation of Extracellular Vesicles with Collagen Hydrogels
  • 07:39Results: Analysis of Vascular Calcification in Collagen Hydrogels
  • 08:23Conclusion

Summary

Traduction automatique

Traduction automatique

This paper presents the methodology for obtaining and assessing vascular calcification by isolating murine aortas followed by extracting calcified extracellular vesicles to observe the mineralization potential.

Tags

Extracellular VesicleVascular CalcificationIn Vitro ModelIn Vivo ModelVascular Smooth Muscle CellCardiovascular DiseaseTherapeutic OptionsMouse DissectionOrgan ExtractionAortaHeartPBS Perfusion

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