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Assessment of Chemical Toxicity in Adult Drosophila Melanogaster
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Biologie
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Journal JoVE Biologie
Assessment of Chemical Toxicity in Adult Drosophila Melanogaster

Assessment of Chemical Toxicity in Adult Drosophila Melanogaster

DOI:
10.3791/65029-v

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07:02 min

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March 24, 2023

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, , , , , , ,
1Department of Biology,Indiana University, 2Bloomington Drosophila Stock Center, Department of Biology,Indiana University, 3School of Biosciences,University of Birmingham, 4O’Neill School of Public and Environmental Affairs,Indiana University, 5Section of Developmental Genomics, Laboratory of Biochemistry and Genetics, National Institute of Diabetes and Kidney and Digestive Diseases,National Institutes of Health

Chapitres

  • 00:04Introduction
  • 00:29Vial and Humidity Chamber Preparation
  • 01:20Preparation of Flies for Chemical Exposure
  • 02:34Fly Chemical Exposure ‐ Range Finding Experiment
  • 04:51Results: Effects of Chemical Toxicants on the Viability of Adult Drosophila Melanogaster
  • 06:29Conclusion

Summary

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This protocol describes an efficient and inexpensive method that uses liquid media to assess the effects of chemical toxicants on the viability of adult Drosophila melanogaster.

Tags

Keywords: Drosophila MelanogasterChemical Toxicity AssessmentNAMsLiquid MediaFilter Paper InsertsStarvation VialsHumidity ChamberChemical Exposure VialsFemale FliesBlue Dye

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