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Determination of Self-(In)compatibility and Inter-(In)compatibility Relationships in Citrus Using Manual Pollination, Microscopy, and S-Genotype Analyses
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Journal JoVE Biologie
Determination of Self-(In)compatibility and Inter-(In)compatibility Relationships in Citrus Using Manual Pollination, Microscopy, and S-Genotype Analyses

Determination of Self-(In)compatibility and Inter-(In)compatibility Relationships in Citrus Using Manual Pollination, Microscopy, and S-Genotype Analyses

DOI:
10.3791/65056-v

•

07:12 min

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June 30, 2023

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, , , , , ,
1National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops,Huazhong Agricultural University

Chapitres

  • 00:05Introduction
  • 01:02Pollen Collection
  • 01:40In Vitro Pollen Germination Test
  • 02:27Pollination
  • 03:02Sampling, Fixation, and Preservation
  • 03:55Aniline Blue Staining
  • 04:46Fluorescence Microscopy
  • 05:26Results: Determining Pollen Compatibility and Incompatibility in Citrus Cultivars
  • 06:16Conclusion

Summary

Traduction automatique

Traduction automatique

This protocol provides a rapid method for determining pollen compatibility and incompatibility in citrus cultivars.

Tags

Self-incompatibilityInter-incompatibilityCitrusManual PollinationMicroscopyS-genotype AnalysisPollen CollectionPollen ViabilityPollinationBreeding Programs

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