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Karakterisering af epitelsårheling in vivo ved hjælp af Cnidarian Model Organism Clytia hemisphaerica
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Biologie
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Journal JoVE Biologie
Characterizing Epithelial Wound Healing In Vivo Using the Cnidarian Model Organism Clytia hemisphaerica

Karakterisering af epitelsårheling in vivo ved hjælp af Cnidarian Model Organism Clytia hemisphaerica

DOI:
10.3791/65081-v

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07:47 min

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February 10, 2023

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, ,
1Department of Molecular Genetics and Cell Biology,The University of Chicago

Chapitres

  • 00:05Introduction
  • 00:53Procedure for Wounding: Microwounds and Small Wounds
  • 02:13Procedure for Wounding: Large Wounds
  • 03:24Imaging Epithelial Wound Healing
  • 03:52Procedure for Mesogleal Injections
  • 05:32Results: The Epithelial Wound Healing Process
  • 07:08Conclusion

Summary

Traduction automatique

Traduction automatique

Dette papir beskriver en metode til at skabe sår i epitelet af en levende Clytia hemisphaerica medusa og billedsårheling ved en høj opløsning in vivo. Derudover præsenteres en teknik til at introducere farvestoffer og lægemidler til forstyrrelsessignalprocesser i epitelcellerne og ekstracellulær matrix under sårheling.

Tags

Keywords: Epithelial Wound HealingClytia HemisphaericaIn VivoLive ImagingMicro-woundsScratch WoundsMicroinjectionCell MigrationCell SpreadingExtracellular MatrixCell Cytoplasm

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