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Semi-Automated Phenotypic Analysis of Functional 3D Spheroid Cell Cultures
Journal JoVE
Biochimie
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Journal JoVE Biochimie
Semi-Automated Phenotypic Analysis of Functional 3D Spheroid Cell Cultures

Semi-Automated Phenotypic Analysis of Functional 3D Spheroid Cell Cultures

DOI:
10.3791/65086-v

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06:48 min

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August 18, 2023

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, , , , ,
1Department of Biochemistry,Albert Einstein College of Medicine, 2Department of Biochemistry and Molecular Biology,University of Southern Denmark, 3CelVivo ApS

Chapitres

  • 00:04Introduction
  • 00:36Preparation of Spheroids and Spheroid Culture in Bioreactor
  • 03:00Imaging and Collection of Spheroids
  • 04:28Proteomics Analysis via Liquid Chromatography Coupled with Mass Spectrometry
  • 05:17Results: Proteome Analysis of Liver Spheroids
  • 06:15Conclusion

Summary

Traduction automatique

Traduction automatique

We present a protocol for growing high-reproducible spheroids and their phenotypic characterization using image capture and proteomics.

Tags

3D Cell CultureSpheroidHepG2/C3ATHLE-3Ultra-low Attachment PlateBioreactorProteomic AnalysisLC-MSPhenotypic Analysis

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