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Isolation and Culture of Human Adipose-Derived Stem Cells With an Innovative Xenogeneic-Free Method for Human Therapy
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Médecine
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Journal JoVE Médecine
Isolation and Culture of Human Adipose-Derived Stem Cells With an Innovative Xenogeneic-Free Method for Human Therapy

Isolation and Culture of Human Adipose-Derived Stem Cells With an Innovative Xenogeneic-Free Method for Human Therapy

DOI:
10.3791/65104-v

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06:00 min

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February 03, 2023

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, , , , , , ,
1Department of Plastic, Reconstructive and Hand Surgery,Centre Hospitalier Universitaire Vaudois (CHUV, 2Cell and Tissue Engineering Lab,IRCCS Ospedale Galeazzi Sant’Ambrogio, 3Unit of Regenerative Therapy, Plastic, Reconstructive and Hand Surgery, Department of Musculoskeletal Medicine,Lausanne University Hospital, 4Centre for the Cellular Microenvironment,University of Glasgow

Chapitres

  • 00:04Introduction
  • 01:04Human Adipose-Derived Stem Cells (hASCs) Isolation from Adipose Tissue
  • 02:21hASC Culture, Cryopreservation, and Storage
  • 04:25Results: Assessment of Human Platelet Lysate (hPL)-Induced hASCs
  • 05:22Conclusion

Summary

Traduction automatique

Traduction automatique

Xenogeneic (chemical or animal-derived) products introduced in the cell therapy preparation/manipulation steps are associated with an increased risk of immune reactivity and pathogenic transmission in host patients. Here, a complete xenogeneic-free method for the isolation and in vitro expansion of human adipose-derived stem cells is described.

Tags

Keywords: Adipose-derived Stem CellsHuman TherapyXenogeneic-free MethodCell IsolationCell ExpansionPeripheral Nerve RepairCell CultureSterilityMicro DissectionCell AttachmentCell ConfluencyCell PassageTrypsinizationCell Collection

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