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1Department of Plastic, Reconstructive and Hand Surgery,Centre Hospitalier Universitaire Vaudois (CHUV, 2Cell and Tissue Engineering Lab,IRCCS Ospedale Galeazzi Sant’Ambrogio, 3Unit of Regenerative Therapy, Plastic, Reconstructive and Hand Surgery, Department of Musculoskeletal Medicine,Lausanne University Hospital, 4Centre for the Cellular Microenvironment,University of Glasgow
Chapitres
- 00:04Introduction
- 01:04Human Adipose-Derived Stem Cells (hASCs) Isolation from Adipose Tissue
- 02:21hASC Culture, Cryopreservation, and Storage
- 04:25Results: Assessment of Human Platelet Lysate (hPL)-Induced hASCs
- 05:22Conclusion
Xenogene (kemiske eller animalsk afledte) produkter, der introduceres i celleterapiforberedelses-/manipulationstrinnene, er forbundet med en øget risiko for immunreaktivitet og patogen overførsel hos værtspatienter. Her beskrives en komplet xenogenfri metode til isolering og in vitro-ekspansion af humane fedtafledte stamceller.
