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1Department of Plastic, Reconstructive and Hand Surgery,Centre Hospitalier Universitaire Vaudois (CHUV, 2Cell and Tissue Engineering Lab,IRCCS Ospedale Galeazzi Sant’Ambrogio, 3Unit of Regenerative Therapy, Plastic, Reconstructive and Hand Surgery, Department of Musculoskeletal Medicine,Lausanne University Hospital, 4Centre for the Cellular Microenvironment,University of Glasgow
Chapitres
- 00:04Introduction
- 01:04Human Adipose-Derived Stem Cells (hASCs) Isolation from Adipose Tissue
- 02:21hASC Culture, Cryopreservation, and Storage
- 04:25Results: Assessment of Human Platelet Lysate (hPL)-Induced hASCs
- 05:22Conclusion
Xenogene (kjemiske eller dyreavledede) produkter introdusert i celleterapiforberedelses-/manipulasjonstrinnene er forbundet med økt risiko for immunreaktivitet og patogen overføring hos vertspasienter. Her beskrives en komplett xenogenfri metode for isolering og in vitro-ekspansjon av humane fettderiverte stamceller.
