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Forstyrrelse av blod-ryggmargsbarrieren ved bruk av lavintensitetsfokusert ultralyd i en rottemodell
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1Department of Neurosurgery,Johns Hopkins University School of Medicine, 2Department of Biomedical Engineering,Johns Hopkins University, 3HEPIUS Innovation Laboratory,Johns Hopkins University School of Medicine, 4Department of Electrical Engineering and Computer Science,Johns Hopkins University, 5Department of Mechanical Engineering,Johns Hopkins University, 6Department of Anesthesiology and Critical Care Medicine,Johns Hopkins University
Chapitres
- 00:04Introduction
- 01:11Low‐Intensity Focused Ultrasound Assembly and Setup
- 02:20Animal Preparation and Surgical Laminectomy
- 03:38Target Localization Using Laser Guidance
- 04:22Microbubble Administration
- 05:27Spinal Cord Extraction, Tissue Processing, and Visualization of BSCB Disruption
- 06:34Results: Confirmation of Target Localization and Evaluating BSCB Disruption by EBD Extravasation into the Spinal Cord
- 08:17Conclusion
Forstyrrelse av blod-ryggmargsbarrieren (BSCB) kan oppnås med intravenøs administrering av mikrobobler og anvendelse av lavintensitetsfokusert ultralyd (LIFU). Denne protokollen beskriver åpningen av BSCB ved bruk av LIFU i en gnagermodell, inkludert utstyrsoppsett, mikrobobleinjeksjon, mållokalisering og BSCB-forstyrrelsesvisualisering.
