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A Mouse Ear Model for Allergic Contact Dermatitis Evaluation
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Journal JoVE Immunologie et infection
A Mouse Ear Model for Allergic Contact Dermatitis Evaluation

A Mouse Ear Model for Allergic Contact Dermatitis Evaluation

DOI:
10.3791/65120-v

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08:02 min

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March 24, 2023

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, , ,
1Department of Traditional Chinese Medicine, The Affiliated Hospital of Yangzhou University,Yangzhou University, 2Department of Traditional Chinese and Western Medicine, Medical College,Yangzhou University, 3The Key Laboratory of Syndrome Differentiation and Treatment of Gastric Cancer of the State Administration of Traditional Chinese Medicine,Yangzhou University, 4Jiangsu Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Senile Diseases,Yangzhou University

Chapitres

  • 00:04Introduction
  • 00:55Animal Preparation
  • 01:35Abdominal Sensitization Stimulation
  • 02:46Ear Sensitization Stimulation
  • 03:38Recording Mouse Weight and Allergic Contact Dermatitis (ACD) Symptoms
  • 04:16Measurement of the Auricle Thickness
  • 04:50Evaluation of the Degree of Inflammatory Swelling
  • 05:59Results: Evaluating the Severity of Allergic Contact Dermatitis
  • 07:32Conclusion

Summary

Traduction automatique

Traduction automatique

Here, we describe the methods for inducing allergic contact dermatitis in mouse ears by 1-fluoro-2,4-dinitrobenzene (DNFB) and how to evaluate the severity of allergic contact dermatitis.

Tags

Keywords: Mouse Ear ModelAllergic Contact DermatitisDNFBSensitizationEar StimulationWeight ChangesPreclinical EvaluationAnimal Model

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