Journal
/
Bio-ingénierie
/
Bioorthogonal Chemical Imaging of Cell Metabolism Regulated by Aromatic Amino Acids
Journal JoVE
Bio-ingénierie
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Bio-ingénierie
Bioorthogonal Chemical Imaging of Cell Metabolism Regulated by Aromatic Amino Acids

Bioorthogonal Chemical Imaging of Cell Metabolism Regulated by Aromatic Amino Acids

DOI:
10.3791/65121-v

•

10:42 min

•

May 12, 2023

•

, , , , ,
1Shu Chien-Gene Lay Department of Bioengineering,University of California San Diego

Chapitres

  • 00:05Introduction
  • 00:54Media Preparation
  • 02:10Cell Sample Preparation
  • 05:22Spontaneous Raman Spectroscopy Measurement
  • 06:15Imaging Experiments with Two-Photon Excitation Fluorescence and Stimulated Raman Scattering
  • 08:25Results: Visualizing Metabolic Dynamics and 3D Lipid Droplet Distribution in HeLa Cells
  • 09:48Conclusion

Summary

Traduction automatique

Traduction automatique

We present a protocol to directly visualize metabolic activities in cells regulated by amino acids using deuterium-oxide (heavy water D2O) probed stimulated Raman scattering (DO-SRS) microscopy, which is integrated with two-photon excitation fluorescence microscopy (2PEF).

Tags

Keywords: BioorthogonalChemical ImagingCell MetabolismAromatic Amino AcidsRaman ImagingTwo-photon FluorescenceSecond Harmonic GenerationDMEMDeuterium OxidePhenylalanineTryptophanMethionineThreoninePoly-D-LysineLamininTrypsin

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/65121?article_type=v

High Resolution 3D Imaging of Ex-Vivo Biological Samples by Micro CT
check_url/fr/65121?article_type=v

Analyzing Cellular Internalization of Nanoparticles and Bacteria by Multi-spectral Imaging Flow Cytometry
check_url/fr/65121?article_type=v

In vitro Cell Culture Model for Toxic Inhaled Chemical Testing
check_url/fr/65121?article_type=v

Harnessing the Bioorthogonal Inverse Electron Demand Diels-Alder Cycloaddition for Pretargeted PET Imaging
check_url/fr/65121?article_type=v

Hand-held Clinical Photoacoustic Imaging System for Real-time Non-invasive Small Animal Imaging
check_url/fr/65121?article_type=v

Engineering ‘Golden’ Fluorescence by Selective Pressure Incorporation of Non-canonical Amino Acids and Protein Analysis by Mass Spectrometry and Fluorescence
check_url/fr/65121?article_type=v

Antimicrobial Peptides Produced by Selective Pressure Incorporation of Non-canonical Amino Acids
check_url/fr/65121?article_type=v

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers
check_url/fr/65121?article_type=v

Visualizing Adhesion Formation in Cells by Means of Advanced Spinning Disk-Total Internal Reflection Fluorescence Microscopy
check_url/fr/65121?article_type=v

Detection of Endotoxin in Nano-formulations Using Limulus Amoebocyte Lysate (LAL) Assays

Read Article