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Human Saphenous Vein Endothelial Cell Isolation and Exposure to Controlled Levels of Shear Stress and Stretch
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Médecine
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Journal JoVE Médecine
Human Saphenous Vein Endothelial Cell Isolation and Exposure to Controlled Levels of Shear Stress and Stretch

Human Saphenous Vein Endothelial Cell Isolation and Exposure to Controlled Levels of Shear Stress and Stretch

DOI:
10.3791/65122-v

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09:10 min

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April 21, 2023

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, , , , , ,
1Instituto do Coraçao (InCor), Hospital das Clinicas HCFMUSP, Faculdade de Medicina,Universidade de Sao Paulo, 2Cardiology Research Group, Faculty VI Medicine and Health Sciences,Carl von Ossietzky University of Oldenburg

Chapitres

  • 00:04Introduction
  • 01:18Isolation and Culture of Primary Human Saphenous Vein Endothelial Cells (hSVECs)
  • 03:37Applying Shear Stress on Human SVECs
  • 05:42Applying Cyclic Stretch on Human SVECs
  • 07:20Results: Effects of Mechanical Stress in Human SVECs
  • 08:34Conclusion

Summary

Traduction automatique

Traduction automatique

We describe a protocol to isolate and culture human saphenous vein endothelial cells (hSVECs). We also provide detailed methods to produce shear stress and stretch to study mechanical stress in hSVECs.

Tags

Saphenous VeinEndothelial Cell IsolationShear StressStretchCoronary Artery Bypass SurgeryMechanical StressCell CultureCollagenaseSmooth Muscle CellsFibroblastsGrowth FactorsPBSCell MediumCentrifugationCell PelletGelatin CoatingCell Culture Dish

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