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Isolation, Culture, and Characterization of Primary Dermal Fibroblasts from Human Keloid Tissue
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Médecine
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Journal JoVE Médecine
Isolation, Culture, and Characterization of Primary Dermal Fibroblasts from Human Keloid Tissue

Isolation, Culture, and Characterization of Primary Dermal Fibroblasts from Human Keloid Tissue

DOI:
10.3791/65153-v

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04:41 min

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July 28, 2023

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, , , , ,
1Dermatology Hospital,Southern Medical University

Chapitres

  • 00:04Introduction
  • 00:35Tissue Preparation and Isolation
  • 01:33Tissue Culture and Identification of Fibroblasts by Immunofluorescence Staining
  • 03:29Results: Characterized Fibroblastic Growth
  • 04:07Conclusion

Summary

Traduction automatique

Traduction automatique

This study describes an optimized protocol for establishing primary fibroblasts from keloid tissues that can effectively and steadily provide pure and viable fibroblasts.

Tags

Keywords: KeloidFibroblastsPrimary Cell CultureImmunofluorescenceDAPICentrifugationTrypsin-EDTADMEMFBSPSA

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