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Osmotic Minipump Implantation for Increasing Glucose Concentration in Mouse Cerebrospinal Fluid
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Journal JoVE Biologie
Osmotic Minipump Implantation for Increasing Glucose Concentration in Mouse Cerebrospinal Fluid

Osmotic Minipump Implantation for Increasing Glucose Concentration in Mouse Cerebrospinal Fluid

DOI:
10.3791/65169-v

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06:21 min

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April 07, 2023

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1Department of Medicine, Division of Endocrinology, Diabetes and Metabolism,University of Rochester School of Medicine and Dentistry

Chapitres

  • 00:04Introduction
  • 01:02Surgery to Implant Osmotic Minipump
  • 02:59Cerebrospinal Fluid (CSF) Collection Procedure
  • 05:11Results: Glucose Infusion in CSF Using the Osmotic Minipump
  • 05:31Conclusion

Summary

Traduction automatique

Traduction automatique

This article describes a detailed protocol to increase glucose concentration in the cerebrospinal fluid (CSF) of mice. This approach can be useful for studying the effects of high CSF glucose on neurodegeneration, cognition, and peripheral glucose metabolism in mice.

Tags

Osmotic Minipump ImplantationCSF Glucose LevelsMouse BrainCognitive DysfunctionNeurodegenerative DisordersStereotaxic SurgeryBregmaDura MaterCannulaDental Cement

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