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In situ Imaging of the Mouse Thymus Using 2-Photon Microscopy
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Journal JoVE Biologie
In situ Imaging of the Mouse Thymus Using 2-Photon Microscopy

In situ Imaging of the Mouse Thymus Using 2-Photon Microscopy

DOI:
10.3791/652-v

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15:00 min

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January 25, 2008

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, ,
1Department of Molecular and Cell Biology,University of California, Berkeley

Chapitres

  • 00:00Titre
  • 00:41Introduction
  • 01:36Adoptive Transfer
  • 02:51Dissection of Thymus
  • 09:01Preparing Thymus for Imaging
  • 10:232-photon Imaging
  • 12:05Example of 2-photon Imaging Results
  • 13:57Conclusion

Summary

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Traduction automatique

We present step-by-step instructions for the generation of neonatal chimeras as well as the dissection and preparation of the thymus for ex vivo imaging by 2-Photon Microscopy.

Tags

In Situ ImagingMouse Thymus2-photon MicroscopyThymocyte DevelopmentMigration Of ThymocytesNeonatal ChimerasHematopoietic ChimerasBone Marrow InjectionThymus DissectionEx Vivo ImagingDMEM Without Phenol RedDiverse T Cell Repertoire

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