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Rat Intestinal Crypts Isolation and Intestinal Organoids Generation

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Генерация и манипуляции с органоидами кишечника крыс

Journal JoVE
Biologie

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Journal JoVE Biologie

Generation and Manipulation of Rat Intestinal Organoids

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Генерация и манипуляции с органоидами кишечника крыс

Rat Intestinal Crypts Isolation and Intestinal Organoids Generation

DOI:

10.3791/65343-v

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01:58 min

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June 23, 2023

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Eleanor Zagoren*¹, Anderson K. Santos*^1,2, Nadia A. Ameen⁴, Kaelyn Sumigray^5,6

¹Department of Genetics,Yale School of Medicine, ²Department of Pediatrics,Yale School of Medicine, ³Department of Pediatrics/Gastroenterology and Hepatology,Yale School of Medicine, ⁴Department of Cellular and Molecular Physiology,Yale School of Medicine, ⁵Yale Stem Cell Center,Yale School of Medicine, ⁶Yale Cancer Center,Yale School of Medicine

Tags

Keywords: Rat Intestinal Organoids Genetic Manipulation Lentiviral Infection Transient Transfection Intestinal Biology Mouse Intestinal Organoids Human Intestinal Organoids Physiological Relevance Disease Modeling Drug Discovery Personalized Treatment Stem Cell Dynamics Cell Fate Decisions

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