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A Nanopore-Based Genomic Approach to Control Rabies in Low-Income Countries

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Sequencing van het hele genoom voor snelle karakterisering van het rabiësvirus met behulp van nanoporietechnologie

Journal JoVE
Biologie

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Journal JoVE Biologie

Whole Genome Sequencing for Rapid Characterization of Rabies Virus Using Nanopore Technology

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Sequencing van het hele genoom voor snelle karakterisering van het rabiësvirus met behulp van nanoporietechnologie

A Nanopore-Based Genomic Approach to Control Rabies in Low-Income Countries

DOI:

10.3791/65414-v

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02:47 min

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August 18, 2023

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Criselda Bautista², Gurdeep Jaswant^3,4,5, Hollie French⁶, Kathryn Campbell, Rowan Durrant, Robert Gifford⁶, Grace S. N. Kia⁸, Brian Ogoti⁹, Katie Hampson, Kirstyn Brunker⁶

¹School of Biodiversity, One Health & Veterinary Medicine,University of Glasgow, ²Research Institute for Tropical Medicine, ³University of Nairobi, Institute of Tropical and Infectious Diseases, ⁴Tanzania Industrial Research Development Organization, ⁵Ifakara Health Institute, ⁶MRC-University of Glasgow Centre for Virus Research, ⁷Department of Veterinary Public health,Ahmadu Bello University, ⁸African Centre of Excellence for Neglected Tropical Diseases and Forensic Biotechnology,Ahmadu Bello University, ⁹University of Nairobi, Kenya and Center for Epidemiological Modelling and Analysis,University of Nairobi

Tags

Whole Genome Sequencing Rabies Virus Nanopore Technology Pathogen Surveillance Low-resource Settings Bioinformatic Tools Viral Genomic Surveillance Epidemiological Insight Dog-mediated Rabies Wildlife Rabies Sample-to-sequence-to-interpretation Workflow

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Sequencing van het hele genoom voor snelle karakterisering van het rabiësvirus met behulp van nanoporietechnologie

Monstervoorbereiding, RNA-extractie, PCR-opschoning en kwantificering voor sequencing van het hele genoom van het rabiësvirus (RABV)

Sequencing, live en offline basecalling, analyse en interpretatie van het genoom van het rabiësvirus (RABV)

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