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Dot-Blot Assay for Detection of Leptospira DNA in Water Samples

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聚合酶链反应和斑点-印迹杂交用于水样中 钩端螺旋体 检测

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Biologie
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Journal JoVE Biologie

Polymerase Chain Reaction and Dot-Blot Hybridization for Leptospira Detection in Water Samples

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聚合酶链反应和斑点-印迹杂交用于水样中钩端螺旋体检测

Dot-Blot Assay for Detection of Leptospira DNA in Water Samples

DOI:

10.3791/65435-v

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00:32 min

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June 14, 2024

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Eduardo Delgadillo-Tellez, Beatriz Zavaleta-Villa, Daniel Atilano-López, Rigoberto Hernández-Castro, Angélica Olivo-Díaz, Erika Margarita Carrillo-Casas

¹Department of Molecular Biology and Histocompatibility,General Hospital “Dr. Manuel Gea González”, ²Diagnostic Laboratory-Bacteriology, Leptospira Section, Department of Microbiology and Immunology, Veterinary Medicine Faculty,UNAM, ³Department of Ecology of Pathogens,General Hospital “Dr. Manuel Gea González”

Tags

Polymerase Chain Reaction Dot-Blot Hybridization Leptospira Water Samples DNA Detection Non-Isotopic Technique Probe Hybridization Nylon Membrane Anti-Digoxigenin Antibody PCR-Dot-Blot Assay Water Concentration Ecosystem Analysis

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