/

/

Centromere-Associated Protein-E Gene Editing Using the CRISPR/Cas9 System

/

Author Spotlight: Establishing CENP-E Knockout HeLa Cells – A Novel Approach to Study Kinesin-7 CENP-E Biology and its Inhibitors

Journal JoVE
Biologie
Author Produced

Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.

Journal JoVE Biologie

Generation of Centromere-Associated Protein-E CENP-E^-/- Knockout Cell Lines using the CRISPR/Cas9 System

Author Spotlight: Establishing CENP-E Knockout HeLa Cells – A Novel Approach to Study Kinesin-7 CENP-E Biology and its Inhibitors

Centromere-Associated Protein-E Gene Editing Using the CRISPR/Cas9 System

DOI:

10.3791/65476-v

•

02:19 min

•

June 23, 2023

•

Meng-Fei Xu², Jie Chen², Yue Xu², Jing-Lian Zhang², Yi Zhou², Jie-Jie He², Shan Wu², Ya-Lan Wei⁴, Zhen-Yu She²

¹Department of Cell Biology and Genetics, The School of Basic Medical Sciences,Fujian Medical University, ²Key Laboratory of Stem Cell Engineering and Regenerative Medicine,Fujian Province University, ³College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics,Fujian Medical University, ⁴Medical Research Center,Fujian Maternity and Child Health Hospital

Tags

Keywords: CENP-E CRISPR/Cas9 Knockout HeLa Cells Chromosome Alignment Mitotic Defects Cell Cycle Arrest Cell Death Kinetochore-microtubule Capture Spindle Assembly Checkpoint

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

Author Spotlight: Establishing CENP-E Knockout HeLa Cells – A Novel Approach to Study Kinesin-7 CENP-E Biology and its Inhibitors

Transfection, Isolation, and Screening of the CENP-E Knockout HeLa Cells

Immunofluorescence Staining and High-Resolution Confocal Microscopy of CRISPR/Cas9-Mediated CENP-E Knockout HeLa Cells

Chromosome Preparation and Karyotype Analysis of CRISPR/Cas9-Mediated CENP-E Knockout HeLa Cells

Read Article