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A Protocol to Isolate, Culture, and Identify Human Limbal Niche Cells

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Standardizing Limbal Niche Cell (LNC) Isolation and Characterization to Support Widespread LNC Research (Video) | JoVE

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Isolation and Identification of Limbal Niche Cells

Standardizing Limbal Niche Cell (LNC) Isolation and Characterization to Support Widespread LNC Research (Video) | JoVE

A Protocol to Isolate, Culture, and Identify Human Limbal Niche Cells

DOI:

10.3791/65618-v

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01:28 min

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October 27, 2023

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Guanyu Su, Wei Wang, Lingjuan Xu, Rong Liu, Yongyao Tan, Bihui Jin, Chunxiu You, Xiao Zhou, Yihong Xiong, Huatao Xie, Guigang Li

¹Department of Ophthalmology, Tongji Hospital, Tongji Medical College,Huazhong University of Science and Technology, ²Institute of Medicine Nursing,Hubei University of Medicine, ³Department of Ophthalmology, Union Hospital, Tongji Medical College,Huazhong University of Science and Technology

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Keywords: Limbal Niche Cells Limbal Stem Cell Deficiency Single Cell Sequencing Mesenchymal Stem Cells Collagenase A Trypsin-EDTA Modified Embryonic Stem Cell Medium Matrigel Immunofluorescence Real-time Quantitative PCR Flow Cytometry Stem Cell Markers VIM CD90 CD105 PDGFR-beta Pan-CK CD73 SCF

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Standardizing Limbal Niche Cell (LNC) Isolation and Characterization to Support Widespread LNC Research (Video) | JoVE

Isolation of Limbal Niche Cells (LNCs) from Limbus Tissue (Video) | JoVE

Identification of Limbal Niche Cells (LNCs) (Video) | JoVE

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