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Journal
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Biologie
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Coating TEM Grids with a Graphene Monolayer
/
Fabrication of Monolayer Graphene-Coated Grids for Cryoelectron Microscopy(초저온 전자 현미경을 위한 단층 그래핀 코팅 그리드 제작)
Journal JoVE
Biologie
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Journal JoVE
Biologie
Fabrication of Monolayer Graphene-Coated Grids for Cryoelectron Microscopy
Please note that all translations are automatically generated.
Click here for the English version.
Fabrication of Monolayer Graphene-Coated Grids for Cryoelectron Microscopy(초저온 전자 현미경을 위한 단층 그래핀 코팅 그리드 제작)
Coating TEM Grids with a Graphene Monolayer
DOI:
10.3791/65702-v
•
01:52 min
•
September 08, 2023
•
Benjamin Basanta*
1
,
Wenqian Chen*
1
,
Daniel E. Pride
,
Gabriel C. Lander
1
Department of Structural and Computational Biology
,
Scripps Research
Tags
Cryoelectron Microscopy
Graphene
Monolayer
Sample Preparation
Air-water Interface
Hydrophobic Effect
Denaturation
Aggregation
Complex Dissociation
Preferred Orientation
Background Noise
Protein Concentration
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Fabrication of Monolayer Graphene-Coated Grids for Cryoelectron Microscopy(초저온 전자 현미경을 위한 단층 그래핀 코팅 그리드 제작)
단층 그래핀-구리 시트를 MMA로 코팅하고 그리드를 청소합니다.
격자를 탈이온수 아래의 천공된 트레이로 옮기고, 그래핀을 격자로 옮기고, MMA를 제거하고, 격자를 청소합니다
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