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Generating Human Gastric Organoids from Upper Endoscopy Biopsies

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Author Spotlight: Generation of and Comparison Between Patient-Derived Gastric Organoids from Different Regions of the Stomach

Journal JoVE
Biologie

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Journal JoVE Biologie

Establishment and Characterization of Patient-derived Gastric Organoids from Biopsies of Benign Gastric Body and Antral Epithelium

Author Spotlight: Generation of and Comparison Between Patient-Derived Gastric Organoids from Different Regions of the Stomach

Generating Human Gastric Organoids from Upper Endoscopy Biopsies

DOI:

10.3791/66094-v

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01:22 min

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January 26, 2024

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Kole H. Buckley, Keely A. Beyries, Sandra Ryeom, Sam S. Yoon, Bryson W. Katona

¹Division of Gastroenterology and Hepatology,University of Pennsylvania Perelman School of Medicine, ²Department of Surgery,Columbia University Irving Medical Center

Tags

Gastric Organoids Patient-derived Organoids Gastric Body Gastric Antrum Single-cell Digest Standardized Seeding Gastric Biology Gastric Pathology Hereditary Gastric Cancer CDH1 CTNNA1 BRCA1 BRCA2

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Author Spotlight: Generation of and Comparison Between Patient-Derived Gastric Organoids from Different Regions of the Stomach

Isolating Single Cells from Upper Endoscopy Biopsies Derived from Gastric Patients for Organoid Generation

Embedding Gastric Biopsy-Derived Single Cells in a Basement Membrane Matrix Dome for Organoid Generation

Routine Passaging of Patient-Derived Gastric Organoids Through Fragmentation

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