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Journal
/
Biologie
/
Novel Protocol for Simultaneous Fluorescence and Ultrastructure Preservation
/
Author Spotlight: Advancements in Correlative Light and Electron Microscopy with Fluorescent Protein Preservation
Journal JoVE
Biologie
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Journal JoVE
Biologie
Epon Post Embedding Correlative Light and Electron Microscopy
Author Spotlight: Advancements in Correlative Light and Electron Microscopy with Fluorescent Protein Preservation
Novel Protocol for Simultaneous Fluorescence and Ultrastructure Preservation
DOI:
10.3791/66141-v
•
00:46 min
•
January 12, 2024
•
Shuyuan Wang*
1
,
Haiyan Xiong*
1,2
,
Qiyuan Chang*
1,3
,
Xudong Zhuang*
4,5,6
,
Yaochen Wu
3
,
Xinrui Wang
5,6
,
Congxian Wu
,
Zhifei Fu
7,8
1
Public Technology Service Center
,
Fujian Medical University
,
2
The School of Pharmacy
,
Fujian Medical University
,
3
The School of Basic Medical Sciences
,
Fujian Medical University
,
4
College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics
,
Fujian Medical University
,
5
Medical Research Center
,
Fujian Maternity and Child Health Hospital
,
6
NHC Key Laboratory of Technical Evaluation of Fertility Regulation for Non-human Primate
,
Fujian Maternity and Child Health Hospital
,
7
Institute of Neuroscience
,
Fujian Medical University
,
8
Fujian Key Laboratory of Molecular Neurology
,
Fujian Medical University
Tags
Correlative Light And Electron Microscopy
CLEM
Fluorescence Microscopy
Electron Microscopy
Ultrastructure
Epon Embedding
MEosEM
Image Alignment
Image Registration
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Author Spotlight: Advancements in Correlative Light and Electron Microscopy with Fluorescent Protein Preservation
Ultrathin Sectioning for Epon Post-Embedding Correlative Light and Electron Microscopy (CLEM)
Creating the Navigation Map for CLEM and Preparation of the Ultrathin Sections for Electron Microscopy
Image Analysis of Brightfield and Fluorescence Images of the Mouse Brain Sections for Epon Post-Embedding Correlative Light and Electron Microscopy
Registration of the Fluorescence Microscopy Image with the Electron Microscopy Image for Correlative Light and Electron Microscopy
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