Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
1Biomedical Engineering Department,Cornell University, 2Neurosurgical Laboratory for Translational Stem Cell Research, Weill Cornell Brain Tumor Center,Weill Cornell Medical College of Cornell University, 3Cell Morphology Department,Instituto de Investigacion Principe Felipe, 4Department of Chemical and Biomolecular Engineering,Cornell University
Chapitres
- 00:30Introduction
- 02:10Assembling the device
- 07:54Cell seeding and imaging
- 11:12Conclusion
We demonstrate that the over expression of epidermal growth factor receptors (EGFR) enhances the motility of neural stem cells(NSCs) using a novel agarose gel based microfluidic device. This technology can be readily adaptable to other mammalian cell systems where cell sources are scarce, such as human neural stem cells, and the turn around time is critical.
Tags
Neural Stem CellsMotilityAgarose Gel-based Microfluidic DeviceMicrofluidic TechnologyCell-based AssaysMicroenvironmentConventional Microfabrication TechniquesMaterialsAgarose GelCell MigrationParallel Microfluidic ChannelsPeristaltic PumpObservationChemical EnvironmentOptical MonitoringExperimental Results
