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Preparação de Gene Gun Bullets e Transfection biobalística de neurônios em cultura Slice
Journal JoVE
Biologie
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Journal JoVE Biologie
Preparation of Gene Gun Bullets and Biolistic Transfection of Neurons in Slice Culture
DOI:

17:42 min

February 13, 2008

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Chapitres

  • 00:02Titre
  • 01:02Introduction
  • 01:51Preparing DNA-Coated Gold Beads
  • 05:36Coating Plastic Tubing
  • 08:51Cutting the Plastic Tubing
  • 10:18Shooting Tissue Slices
  • 14:20Résultats
  • 15:53Troubleshooting
  • 17:04Conclusion

Summary

Traduction automatique

Nós descrevemos um método para preparar balas DNA revestido de ouro e demonstrar o uso de balas tais biolistically transfectar neurônios em cultura de fatias do hipocampo.

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