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Labeling hESCs and hMSCs with Iron Oxide Nanoparticles for Non-Invasive in vivo Tracking with MR Imaging
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Journal JoVE Biologie
Labeling hESCs and hMSCs with Iron Oxide Nanoparticles for Non-Invasive in vivo Tracking with MR Imaging

Labeling hESCs and hMSCs with Iron Oxide Nanoparticles for Non-Invasive in vivo Tracking with MR Imaging

DOI:
10.3791/685-v

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09:06 min

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March 31, 2008

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, ,
1Contrast Agent Research Group at the Center for Molecular and Functional Imaging, Department of Radiology, University of California San Francisco

Chapitres

  • 00:01Titre
  • 02:03Labeling of Human Mesenchymal Stem Cells with Ferucarbotran
  • 04:21Labeling of Human Embryonic Stem Cell with Ferumoxides
  • 07:58Imaging Stem Cells in vivo
  • 08:23Conclusion

Summary

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For the evaluation of new stem cell therapies it is important to non-invasively track the injected cells in vivo. This video will show you how to label human mesenchymal and embryonic stem cells with iron oxide based contrast agents in vivo for subsequent MR imaging in vivo.

Tags

LabelingHESCsHMSCsIron Oxide NanoparticlesNon-invasiveIn Vivo TrackingMR ImagingStem Cell ResearchBiologie du développementRegenerative MedicineTransplanted Stem CellsMechanismsStem Cell DeathTrophic FactorsSignaling PathwaysStem Cell EngraftmentMR Contrast AgentsSuperparamagnetic Iron Oxide Particles (SPIO)BiocompatibilityDextranCarboxydextranStarch Coat

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