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Whole-cell Recordings of Light Evoked Excitatory Synaptic Currents in the Retinal Slice
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Journal JoVE Biologie
Whole-cell Recordings of Light Evoked Excitatory Synaptic Currents in the Retinal Slice

Whole-cell Recordings of Light Evoked Excitatory Synaptic Currents in the Retinal Slice

DOI:
10.3791/771-v

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19:33 min

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July 02, 2008

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, ,
1Program in Neuroscience,Boston University, 2Department of Biology,Boston University, 3Department of Biomedical Engineering,Boston University

Chapitres

  • 00:00Titre
  • 00:10Preparations for the Dissection
  • 02:40Dissection of the Eye
  • 09:42Moving Slices to the Recording Chamber
  • 12:08Preparation of patch Electrodes
  • 13:21The Recording Rig
  • 14:09Whole-cell Recording
  • 17:40Recording Light-evoked Currents
  • 18:55Discussion/ Conclusion

Summary

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This video shows the process of whole-cell voltage clamp recordings in the retinal slice of the aquatic tiger salamander. We demonstrate the preparation of the slice as well as how to perform patch clamp recordings during visual stimulation of the retina.

Tags

Whole-cell RecordingsLight Evoked Excitatory Synaptic CurrentsRetinal SliceWhole-cell Patch Clamp TechniqueVisual Information ProcessingRetinaAquatic Tiger SalamanderAnimal ModelEye DissectionElectrophysiological RecordingsRecording ChamberWhole-cell Voltage Clamp RecordingsVisual StimuliPhotoreceptorsLight-evoked Current ResponsesPharmacological Agents8-channel Perfusion SystemRetinal Slice PreparationPatch Clamp RecordingsAmacrine CellsBipolar Cells

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