Journal
/
Biologie
/
In vitro Differentiation of Mouse Embryonic Stem (mES) Cells Using the Hanging Drop Method
Journal JoVE
Biologie
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Biologie
In vitro Differentiation of Mouse Embryonic Stem (mES) Cells Using the Hanging Drop Method

In vitro Differentiation of Mouse Embryonic Stem (mES) Cells Using the Hanging Drop Method

DOI:
10.3791/825-v

•

07:07 min

•

July 23, 2008

•

,
1Division of Cardiovascular Medicine,Stanford University

Chapitres

  • 00:00Titre
  • 01:10Introduction
  • 02:06Preparing Single Cell Suspension of Embryonic Stem Cells
  • 03:46Generating Hanging Drop Cultures
  • 04:39Transferring Hanging Drops to Ultralow Attachment Plates
  • 05:17Plating Embryoid Bodies onto Gelatin- Coated Plates
  • 06:12Conclusion

Summary

Traduction automatique

Traduction automatique

This video demonstrates how to conduct in vitro differentiation of mouse embryonic stem cells to embryoid bodies using the hanging drop method.

Tags

In Vitro DifferentiationMouse Embryonic Stem CellsHanging Drop MethodStem Cell DivisionSpecialized Cell TypesCell-based TherapiesSuspension CultureEmbryoid BodiesAggregation Of ES CellsHanging Drop CulturePetri Dish Lid

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/825?article_type=v

Patterning of Embryonic Stem Cells Using the Bio Flip Chip
check_url/fr/825?article_type=v

In vitro and in vivo Bioluminescence Reporter Gene Imaging of Human Embryonic Stem Cells
check_url/fr/825?article_type=v

In vitro Labeling of Human Embryonic Stem Cells for Magnetic Resonance Imaging
check_url/fr/825?article_type=v

The Preparation of Drosophila Embryos for Live-Imaging Using the Hanging Drop Protocol
check_url/fr/825?article_type=v

Isolation and Derivation of Mouse Embryonic Germinal Cells
check_url/fr/825?article_type=v

Differentiation of Embryonic Stem Cells into Oligodendrocyte Precursors
check_url/fr/825?article_type=v

Effektiv Differensiering av mus embryonale stamceller i motoriske nevroner
check_url/fr/825?article_type=v

Generasjon Mus Utledet fra Utførte pluripotente stamceller
check_url/fr/825?article_type=v

Differensiering av nyfødte mus Skin Avledet stamceller inn Germ-lignende celler<em> In vitro</em
check_url/fr/825?article_type=v

Profilering Individuell humane embryonale stamceller ved kvantitativ RT-PCR

Read Article