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Screening for Amyloid Aggregation by Semi-Denaturing Detergent-Agarose Gel Electrophoresis
Journal JoVE
Biologie
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Journal JoVE Biologie
Screening for Amyloid Aggregation by Semi-Denaturing Detergent-Agarose Gel Electrophoresis

Screening for Amyloid Aggregation by Semi-Denaturing Detergent-Agarose Gel Electrophoresis

DOI:
10.3791/838-v

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10:03 min

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July 16, 2008

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,
1Whitehead Institute for Biomedical Research, 2Department of Biology,MIT – Massachusetts Institute of Technology, 3Howard Hughes Medical Institute

Chapitres

  • 00:00Titre
  • 00:48Introduction
  • 01:41Preparing the Gel
  • 03:10Preparing The Samples
  • 06:23Capillary Gel Transfer
  • 09:15Typical Blotting Results
  • 09:58Conclusion

Summary

Traduction automatique

Traduction automatique

SDD-AGE is a useful technique for the detection and characterization of amyloid-like polymers in cells. Here we demonstrate an adaptation that makes this technique amenable to large-scale applications.

Tags

Amyloid AggregationProtein MisfoldingPrionsConformationally Self-templating ProteinsLower OrganismsInsolubilityStructural HeterogeneitySemi-Denaturing Detergent-Agarose Gel Electrophoresis (SDD-AGE)Size-based ResolutionDetergent InsolubilityAmyloid PolymersDetectionCharacterizationConformational VariantsLarge-scale ApplicationsScreens For Prions And Amyloidogenic ProteinsSDD-AGE MethodCapillary TransferReliabilityEase Of UseSDS-insoluble Conformers

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