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The MODS method for diagnosis of tuberculosis and multidrug resistant tuberculosis
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Journal JoVE Biologie
The MODS method for diagnosis of tuberculosis and multidrug resistant tuberculosis

The MODS method for diagnosis of tuberculosis and multidrug resistant tuberculosis

DOI:
10.3791/845-v

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23:06 min

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August 11, 2008

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, , ,
1The Warren Alpert Medical School of Brown University, 2Laboratorio de Investigacion de Enfermedades Infecciosas,Universidad Peruana Cayetano Heredia, 3Department of International Health,Johns Hopkins Bloomberg School of Public Health, 4Wellcome Trust Centre for Clinical Tropical Medicine,Imperial College London

Chapitres

  • 00:13Introduction
  • 03:18Equipment
  • 04:53Reagents
  • 06:20Stock solutions
  • 09:18Working solutions
  • 10:37Antibiotic working solutions
  • 11:19Decontamination
  • 13:15Adding antibiotics to samples
  • 14:19Quality control
  • 16:18Plate reading
  • 19:01Biosafety

Summary

Traduction automatique

Traduction automatique

The microscopic-observation drug-susceptibility (MODS) assay is a low-cost, low-tech tool for high-performance detection of tuberculosis (TB) and multidrug-resistant tuberculosis (MDRTB). This video describes the MODS liquid media culture method.

Tags

MODS MethodDiagnosisTuberculosisMultidrug Resistant TuberculosisActive Pulmonary TuberculosisDrug ResistanceMicroscopic-observation Drug-susceptibility AssayLiquid MediaSolid MediaMycobacterium TuberculosisAtypical MycobacteriaFungal ContaminationBacterial ContaminationIsoniazidRifampicinDirect DetectionSubcultureSensitivitySputum SmearCulture Methods

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