Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
Chapitres
- 00:00Titre
- 00:56Introduction
- 01:20Extraction of Nuclei from Postmortem Human Brain
- 03:34Immunostaining of Purified Nuclei
- 05:54Isolation of Neuronal Nuclei by Fluorescence Activated Sorting
- 08:50Concentration of Neuronal Nuclei by Centrifugation
- 10:08Conclusion
The cellular heterogeneity of brain tissue poses a significant limitation for the study of epigenetic markings in chromatin because most assays lack single cell resolution. Neurons typically are intermingled with glia and other non-neuronal cells. We provide a protocol to extract and collect neuronal nuclei from human brain.
Tags
Neuronal Nuclei IsolationNeuronal ChromatinNuclear OrganizationChromatin And DNA-based AssaysSingle Cell ResolutionCellular HeterogeneityGlia CellsNon-neuronal CellsCell-type Specific CulturesCNS CellsEpigenetic MechanismsHypotonic Lysis BufferUltracentrifugationImmunotaggingAnti-NeuN AntibodyFluorescence-activated SortingChromatin Immunoprecipitation Studies
