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Patch Clamp Recording of Ion Channels Expressed in Xenopus Oocytes
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Journal JoVE Biologie
Patch Clamp Recording of Ion Channels Expressed in Xenopus Oocytes

Patch Clamp Recording of Ion Channels Expressed in Xenopus Oocytes

DOI:
10.3791/936-v

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08:00 min

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October 16, 2008

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, ,
1Department of Molecular and Cellular Physiology,Stanford University , 2Department of Molecular and Cellular Physiology,Stanford University School of Medicine

Chapitres

  • 00:00Titre
  • 00:49Introduction
  • 01:35Removing the Vitelline Membrane
  • 03:33Gigaseal Formation
  • 06:05Outside-Out Topology
  • 06:59Conclusion

Summary

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This is intended as an introduction to patch clamp recording from Xenopus laevis oocytes. It covers vitelline membrane removal, formation of a gigaohm seal (gigaseal), and the optional conversion of the patch to the outside-out topology.

Tags

Patch Clamp RecordingIon ChannelsXenopus OocytesElectrophysiological MeasurementHeterologous CellsAfrican Clawed FrogXenopus LaevisMacropatchSingle-channel RecordingOocyte PreparationSeal GenerationSuccessful Seal Generation

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