Journal
/
Biologie
/
A Technique for Serial Collection of Cerebrospinal Fluid from the Cisterna Magna in Mouse
Journal JoVE
Biologie
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Biologie
A Technique for Serial Collection of Cerebrospinal Fluid from the Cisterna Magna in Mouse

A Technique for Serial Collection of Cerebrospinal Fluid from the Cisterna Magna in Mouse

DOI:
10.3791/960-v

•

08:08 min

•

November 10, 2008

•

,
1Department of Pathology,Columbia University

Chapitres

  • 00:00Titre
  • 01:02Introduction
  • 02:10Making the Glass Capillary
  • 02:52Anesthetizing the Mice
  • 03:19Preparing the Mouse for CSF Sampling
  • 04:42Collecting CSF Using the Cisterna Magna Puncture Technique
  • 06:03After CSF Sampling
  • 06:51Conclusion

Summary

Traduction automatique

Traduction automatique

Transgenic (Tg) mouse models of AD provide an excellent opportunity to investigate how and why Aβ or tau levels in CSF change as the disease progresses in human patients. Here, we demonstrate a refined cisterna magna puncture technique for serial CSF sampling from the mouse.

Tags

Cerebrospinal FluidCSFCisterna MagnaMouseAlzheimer’s DiseaseADNeurodegenerative DiseaseAmyloid PeptideTau ProteinBiochemical ChangesTransgenic Mouse ModelsPuncture TechniqueSamplingAβ42Tau LevelsDisease ProgressionELISAMetabolism

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/960?article_type=v

A 3D System for Culturing Human Articular Chondrocytes in Synovial Fluid
check_url/fr/960?article_type=v

Vampiric Isolation of Extracellular Fluid from Caenorhabditis elegans
check_url/fr/960?article_type=v

Generation of Mice Derived from Induced Pluripotent Stem Cells
check_url/fr/960?article_type=v

Serial Enrichment of Spermatogonial Stem and Progenitor Cells (SSCs) in Culture for Derivation of Long-term Adult Mouse SSC Lines
check_url/fr/960?article_type=v

RNA Isolation from Mouse Pancreas: A Ribonuclease-rich Tissue
check_url/fr/960?article_type=v

The Infiltration-centrifugation Technique for Extraction of Apoplastic Fluid from Plant Leaves Using Phaseolus vulgaris as an Example
check_url/fr/960?article_type=v

The Assembly and Application of ‘Shear Rings’: A Novel Endothelial Model for Orbital, Unidirectional and Periodic Fluid Flow and Shear Stress
check_url/fr/960?article_type=v

The Mouse Isolated Perfused Kidney Technique
check_url/fr/960?article_type=v

Single Cell Collection of Trophoblast Cells in Peri-implantation Stage Human Embryos
check_url/fr/960?article_type=v

Tissue Collection and RNA Extraction from the Human Osteoarthritic Knee Joint

Read Article