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Isolation and Culture of Post-Natal Mouse Cerebellar Granule Neuron Progenitor Cells and Neurons
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Journal JoVE Biologie
Isolation and Culture of Post-Natal Mouse Cerebellar Granule Neuron Progenitor Cells and Neurons

Isolation and Culture of Post-Natal Mouse Cerebellar Granule Neuron Progenitor Cells and Neurons

DOI:
10.3791/990-v

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16:04 min

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January 16, 2009

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, , ,
1Department of Genetics and Development,Columbia University , 2Department of Pathology and Cell Biology,Columbia University , 3Department of Neuroscience,Columbia University , 4Department of Neurology, Beth Israel Deaconess Medical Center,Harvard Medical School

Chapitres

  • 00:00Titre
  • 01:10Introduction
  • 01:37Preparing for Cerebellar Dissection
  • 03:15Cerebellar Dissection and Meninges Removal
  • 06:01Cerebellar Dissection and Meninges Removal
  • 09:29Percoll Gradient Separation
  • 12:03Pre-plating and Plating
  • 13:23Résultats
  • 14:32Conclusion

Summary

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Traduction automatique

Here we present a method to isolate and culture cerebellar granule neuron progenitor cells and cerebellar granule neurons from postnatal mouse.

Tags

IsolationCulturePost-natalMouse CerebellarGranule Neuron Progenitor CellsNeuronsCerebellar CortexNeuronal PropertiesDevelopmentGranule NeuronsPurkinje CellsInhibitory InterneuronsMammalian BrainRodentsExternal Granule Layer (EGL)EnrichmentPurityDifferentiationProliferating Progenitor CellsSynaptogenesisGlutamate Receptor FunctionInteraction With Other Purified Cerebellar CellsCell Death

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