Smith and Nephew View Institution's Website 1 article published in JoVE Bioengineering Induction of Adhesion-dependent Signals Using Low-intensity Ultrasound James Roper1, Andrew Harrison2, Mark D. Bass1 1School of Biochemistry, University of Bristol, 2Smith and Nephew This protocol describes the stimulation of cultured fibroblasts with low-intensity pulsed ultrasound, which drives focal adhesion formation and Rac1 activation by mimicking engagement of the transmembrane matrix receptor, syndecan-4. This approach allows investigation of a successful clinical technique at the cellular level, thereby providing opportunities for refinement of the therapy.