Nanjing Normal University 2 articles published in JoVE Chemistry Capillary Electrophoresis-based Hydrogen/Deuterium Exchange for Conformational Characterization of Proteins with Top-down Mass Spectrometry Lingxiao Chaihu1,2, Xiaopeng Yao1, Xiang Xu1,2, Zhongqin Zhu1, David D. Y. Chen3, Guanbo Wang1 1School of Chemistry and Materials Science, Nanjing Normal University, 2Institute for Cell Analysis, Shenzhen Bay Laboratory, 3Department of Chemistry, University of British Columbia Presented here is a protocol for a capillary electrophoresis-based hydrogen/deuterium exchange (HDX) approach coupled with top-down mass spectrometry. This approach characterizes the difference in higher-order structures between different protein species, including proteins in different states and different proteoforms, by conducting concurrent differential HDX and electrophoretic separation. Biology Luciferase Complementation Imaging Assay in Nicotiana benthamiana Leaves for Transiently Determining Protein-protein Interaction Dynamics Kaiwen Sun*1, Yuyu Zheng*1, Ziqiang Zhu1 1Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University This manuscript describes an easy and rapid experimental procedure for determining protein-protein interactions based on the measurement of luciferase activity.