Jinan University 15 articles published in JoVE Medicine A Rat Dry Eye Model with Lacrimal Gland Dysfunction Induced by Scopolamine Shixu Li1,2, Yu Xiao3, Yan Tang1,2, Yongxin Zhang1,2, Yingge Ma1,2, Ling Wang*1,2, Lin Ye*1,2 1Shenzhen Eye Hospital, Jinan University, 2Shenzhen Eye Institute, 3Shenzhen Research Institute, Wuhan University Here, we establish a rat model of lacrimal gland dysfunction to provide a basis for the study of aqueous-deficient dry eye. Medicine Laparoscopic Common Bile Duct Exploration in Patients with a Previous History of Biliary Tract Surgery Junjie Liang*1, Jiexing Li*1, Yuchuan Jiang1, Qiang Li1, Zhilong Liu1, Leyang Xiang1, Youzhu Hu1,2, Mingrong Cao1 1Department of Hepatobiliary Surgery, The First Affiliated Hospital, Jinan University, 2Department of General Surgery, The Affiliated Shunde Hospital, Jinan University Programmed surgery based on surgical methods and anatomical markers helps shorten the operation time, reduce complications, and improve the safety of the surgery. This study investigated and summarized the surgical methods and anatomic markers of laparoscopic common bile duct exploration in patients with a history of biliary tract surgery. Neuroscience Dual-Task Stroop Paradigm for Detecting Cognitive Deficits in High-Functioning Stroke Patients Songjun Lin1,2, Qiang Lin3,4,5, Biyi Zhao3,4,6, Yongchun Jiang3,4, Wanqi Zhuang3,4, Delong Chen3,4, Yajie Zhang3,4, Aijia Chen3,4, Qianrong Zhang7, Yuxin Zheng3,4, Jianjun Wang1, Fangqiu Xu8, Xi Qin3,4,9, Yefeng Cai10 1Department of Neurology and Psychology, the Fourth Clinical Medical College, Guangzhou University of Chinese Medicine, Shenzhen Traditional Chinese Medicine Hospital, 2Guangzhou University of Chinese Medicine, 3Department of Rehabilitation Medicine, The Fifth Affiliated Hospital of Guangzhou Medical University, 4Department of Rehabilitation Medicine, Guangzhou Medical University, 5Key Laboratory of Biological Targeting Diagnosis, Therapy and Rehabilitation of Guangdong Higher Education Institutes, The Fifth Affiliated Hospital of Guangzhou Medical University, 6School of Traditional Chinese Medicine, Jinan University, 7Health College of Guangdong Pharmaceutical University, 8Department of Clinical Medicine, Guangzhou Medical University, 9Department of Rehabilitation Medicine, Panzhihua Central Hospital, 10Department of Neurology and Psychology, the Second Affiliated Hospital of Guangzhou University of Chinese Medicine Clinical assessment scales are notsensitive enough to cognitive dysfunction in high-functioning stroke patients. The dual-task paradigm presents advantages and potential in the assessment and cognitive training of cognitive dysfunction. The study here proposes a dual-task Stroop paradigm to identify cognitive dysfunction in high-functioning stroke patients. Medicine An Apical Resection Model in the Adult Xenopus tropicalis Heart Si-Yi He*1, Yi-Min Zhou*1, Na Wen1, Ke Meng1, Dong-Qing Cai1, Xu-Feng Qi1 1Key Laboratory of Regenerative Medicine of Ministry of Education, Department of Developmental & Regenerative Biology, Jinan University Xenopus tropicalis is an ideal model for regenerative research as many of its organs possess a remarkable regenerative capacity. Here, we present a method for constructing a heart injury model in X. tropicalis via apex resection. Biochemistry An Integrated Workflow of Identification and Quantification on FDR Control-Based Untargeted Metabolome Dehua Li*1, Junze Liang*1, Yongjian Zhang2, Gong Zhang1,2 1Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes and MOE Key Laboratory of Tumor Molecular Biology, Institute of Life and Health Engineering, College of Life Science and Technology, Jinan University, 2Chi-Biotech Co. Ltd. We constructed an untargeted metabolomic workflow that integrated XY-Meta and metaX together. In this protocol, we displayed how to use XY-Meta to generate a decoy spectral library from open access spectra reference, and then performed FDR control and used the metaX to quantitate the metabolites after identifying the metabolomics spectra. Genetics Investigating the Pathogenesis of MYH7 Mutation Gly823Glu in Familial Hypertrophic Cardiomyopathy using a Mouse Model Yu Xia*1,2, Jinlin Hu*3, Xiang Li4, Shuang Zheng4, Ge Wang1,2, Songtao Tan1,2, Zengxiao Zou1,2, Qiong Ling2,5, Fenghua Yang4, Xiaoping Fan1,2 1Department of Cardiovascular Surgery, Guangdong Provincial Hospital of Traditional Chinese Medicine, the Second Affiliated Hospital of Guangzhou University of Chinese Medicine, 2The Second Clinical College of Guangzhou University of Chinese Medicine, 3Department of Cardiovascular Surgery, The First Affiliated Hospital, Jinan University, 4Guangdong Provincial Key Laboratory of Laboratory Animals, Guangdong Laboratory Animals Monitoring Institute, 5Department of Anesthesiology, Guangdong Provincial Hospital of Chinese Medicine, the Second Affiliated Hospital of Guangzhou University of Chinese Medicine Based on the familial hereditary cardiomyopathy family found in our clinical work, we created a C57BL/6N mouse model with a point mutation (G823E) at the mouse MYH7 locus through CRISPR/Cas9-mediated genome engineering to verify this mutation. Neuroscience Dissecting Cell-Autonomous Function of Fragile X Mental Retardation Protein in an Auditory Circuit by In Ovo Electroporation Qiwei Fan*1, Xiaotan Zhang*2, Yuan Wang3, Xiaoyu Wang1 1Division of Histology & Embryology, Key Laboratory for Regenerative Medicine of the Ministry of Education, College of Medicine, Jinan University, 2Department of Clinical Pathology, First Affiliated Hospital of Jinan University, 3Program in Neuroscience, Department of Biomedical Sciences, College of Medicine, Florida State University Using in ovo electroporation, we devised a method to selectively transfect the auditory inner ear and cochlear nucleus in chicken embryos to achieve a cell-group-specific knockdown of fragile X mental retardation protein during discrete periods of circuit assembly. Behavior Loneliness Assuaged: Eye-Tracking an Audience Watching Barrage Videos Guangyao Chen1, Shuhua Zhou2 1School of Journalism & Communication/National Media & Experimental Teaching Center, Jinan University, 2School of Journalism, University of Missouri The study proposes an activation-match model to study how loneliness is mitigated when a lonely audience watches barrage videos of rational and emotional appeals. The protocol uses eye tracking to document duration and fixation, accounting for the degree of satisfaction when emotional needs are appeased by content and barrage. Genetics A Robust Polymerase Chain Reaction-based Assay for Quantifying Cytosine-guanine-guanine Trinucleotide Repeats in Fragile X Mental Retardation-1 Gene Huilin Wang*1, Xiaofan Zhu*2,3, Baoheng Gui*3,4, Wan Chee Cheung2, Mengmeng Shi2,3, Zhenjun Yang2,3, Ka Yin Kwok2, Ricky Lim5, Sanna Pietilä5, Yuanfang Zhu6,7, Kwong Wai Choy2,3 1Central Laboratory, Bao'an Maternity and Child Health Hospital, Jinan University, 2Department of Obstetrics and Gynaecology, The Chinese University of Hong Kong, 3Shenzhen Research Institute, The Chinese University of Hong Kong, 4Department of Genetics and Metabolism, Maternal and Child Health Hospital of Guangxi Zhuang Autonomous Region, 5PerkinElmer Diagnostics, 6Department of Obstetrics and Gynecology, Bao'an Maternity and Child Health Hospital, Jinan University, 7Maternal-Fetal Medicine Institute, Bao'an Maternity and Child Health Hospital, Jinan University An accurate and robust polymerase chain reaction-based assay for quantifying cytosine-guanine-guanine trinucleotide repeats in the Fragile X mental retardation-1 gene facilitates molecular diagnosis and screening of Fragile X syndrome and Fragile X-related disorders with shorter turn-around time and investment in equipment. Immunology and Infection Renal Subcapsular Transplantation of 2'-Deoxyguanosine-Treated Murine Embryonic Thymus in Nude Mice Jianxue Wang1,3,4, Guolin Chen1,3,4, Qianwen Cui2,3,4, Erfei Song1,3,4, Weihua Tao1, Wanqun Chen2, Cunchuan Wang1,3,4, Shiqi Jia1,3,4 1The First Affiliated Hospital, Jinan University, 2Department of Biochemistry and Molecular Biology, School of Basic Medical Science, Jinan University, 3The Institute of Clinical Medicine, Jinan University, 4Joint institute of Metabolic Medicine Between Jinan University and University of Hong Kong We provide a simple and efficient method to transplant 2’-deoxyguanosine treated E18.5 thymus into the renal capsule of a nude mouse. This method should aide in the study of both thymic epithelial cells function and T cells maturation. Behavior Evaluating the Anti-depression Effect of Xiaoyaosan on Chronically-stressed Mice Zhi-Yi Yan*1, Xiao-Juan Li*1,2, Xiu-Fang Ding1, Yue-Yun Liu1, Jia-xu Chen1,2 1School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, 2Formula-pattern Research Center, School of Traditional Chinese Medicine, Jinan University Here, we present a protocol to establish the mouse depressive model, observe the behavior changes associated with chronic unpredictable mild stress (CUMS), and evaluate the anti-depression effect of Xiaoyaosan. Bioengineering Construction of a Multilayered Mesenchymal Stem Cell Sheet with a 3D Dynamic Culture System Yingwei Wang*1,2, Cheng Lu*1,2, Chengzhi He3, Baoxin Chen1,2, Youling Zheng1,2, Junming Zheng1,2, Jianhua Zhang4, Zheng Wu1,2 1Key Laboratory for Regenerative Medicine, Ministry of Education, Jinan University, 2Department of Developmental and Regenerative Biology, Jinan University, 3Nansha College Preparatory Academy, 4Department of Cardiology, First Affiliated Hospital of Jinan University This article provides an efficient and feasible method for constructing multilayered stem cell sheets with favorable stem cell property. Chemistry Real-time Breath Analysis by Using Secondary Nanoelectrospray Ionization Coupled to High Resolution Mass Spectrometry Xue Li*1,2, Dan D. Huang*3, Rui Du1,2, Zhi J. Zhang1, Chak K. Chan3, Zheng X. Huang1,2, Zhen Zhou1,2 1Institute of Mass Spectrometer and Atmospheric Environment, Jinan University, 2Guangdong Provincial Engineering Research Center for On-line Source Apportionment System of Air Pollution, 3School of Energy and Environment, City University of Hong Kong A protocol for characterizing chemical composition of exhaled breath in real time by using secondary nanoelectrospray ionization coupled to high resolution mass spectrometry is demonstrated. Environment Composition and Properties of Aquafaba: Water Recovered from Commercially Canned Chickpeas Youn Young Shim1,2,3, Rana Mustafa1, Jianheng Shen1, Kornsulee Ratanapariyanuch1, Martin J. T. Reaney1,2,3 1Department of Plant Sciences, University of Saskatchewan, 2Prairie Tide Chemicals Inc., 3Guangdong Saskatchewan Oilseed Joint Laboratory (GUSTO), Department of Food Science and Engineering, Jinan University Aquafaba is a viscous juice from canned chickpea that, when stirred vigorously, produces a relatively stable white froth or foam. The primary research goal is to identify the components of aquafaba that contribute viscosifying/thickening properties using nuclear magnetic resonance (NMR), ultrafiltration, electrophoresis, and peptide mass fingerprinting. Neuroscience Assessment of Neuronal Viability Using Fluorescein Diacetate-Propidium Iodide Double Staining in Cerebellar Granule Neuron Culture Lin Jiajia*1, Mak Shinghung*2, Zheng Jiacheng1, Wang Jialing1, Xu Dilin1, Hu Shengquan2, Zhang Zaijun3, Wang Qinwen1, Han Yifan2,4, Cui Wei1,2 1Ningbo Key Laboratory of Behavioral Neuroscience, Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo University, 2Department of Applied Biology and Chemistry Technology, Institute of Modern Chinese Medicine, The Hong Kong Polytechnic University, 3Institute of New Drug Research, Guangdong Provincial Key Laboratory of Pharmacodynamic, Constituents of Traditional Chinese Medicine & New Drug Research, College of Pharmacy, Jinan University, 4International Joint Laboratory (SYSU-PolyU HK) of Novel Anti-Dementia Drugs of Guangdong This protocol describes how to accurately measure neuronal viability using Fluorescein diacetate (FDA) and Propidium Iodide (PI) double staining in cultured cerebellar granule neurons, a primary neuronal culture used as an in vitro model in neuroscience and neuropharmacology research.
