Sun Yat-sen University Cancer Center 1 article published in JoVE Cancer Research Establishment of Gastric Cancer Patient-derived Xenograft Models and Primary Cell Lines Jia-huan Lu*1, Yun Wang*1, Qi Meng*1, Zhao-lei Zeng1 1State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Sun Yat-sen University Cancer Center The current protocol describes methods to establish patient-derived xenograft (PDX) models and primary cancer cell lines from surgical gastric cancer samples. The methods provide a useful tool for drug development and cancer biology research.